Addgene - pGT2 Plasmid Data

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Plasmid 13056: pGT2

Gene/insert name:		none
Insert size (bp):		Unknown
Vector backbone:		pGT2 (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		3267
Cloning site 5':		XcmI, HindIII, StyI, BspEI, EcoRV
Site destroyed during cloning:		No
Cloning site 3':		XcmI, SacI, SpeI, NotI, EcoRI, AatII
Site destroyed during cloning:		No
5' Sequencing primer:		M13/pUC Reverse (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		Modified from pGFPTA (Husimi et al, 2000)
Sequence:		View sequence
Author's Map:		View map
Principal Investigator:		Chaoyang Zeng
Terms and Licenses:		MTA

Comments: pGT2 is an XcmI generated T-vector bearing GFP marker optimized for direct cloning. Colonies with insert are selected by GFP inactivation.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

lac_promoter

88 - 117

M13_pUC_rev_primer

131 - 153

M13_reverse_primer

152 - 170

GFP_R_primer

292 - 264

GFPfusionREV_primer

327 - 308

ORF frame 3

162 - 956

GFP_cyc3(variant)

234 - 941

GFP_F_primer

887 - 907

AmpR_promoter

1111 - 1139

ORF frame 2

1181 - 2041

Ampicillin

1181 - 2041

pBR322_origin

2196 - 2815

pGEX_3_primer

3212 - 3234

Unique restriction sites

HindIII		179
XbaI		203
NcoI		399
MscI		404
EcoRV		526
XhoI		654
SacI		940
SpeI		952
NotI		963
EagI		963
EcoRI		970
AatII		1049
FspI		1745
PvuII		3267

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13056" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.