Addgene - pEGFP-N1-wtVHL Plasmid Data

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NM_052801

NCBI: Vhl

Vhl antibodies

This is commonly
requested with

pEGFP-C1-wtVHL

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Plasmid 13389: pEGFP-N1-wtVHL

Gene/insert name:		von Hippel-Lindau
Alternative names:		VHL
Insert size (bp):		572
GenBank/Entrez ID of insert:		NM_052801
Gene/insert aliases:		Vhl, Vhlh
Species of gene(s):		R. norvegicus (rat)
Vector backbone:		PEGFP-N1 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression
Backbone size (bp):		4695
Cloning site 5':		HindIII
Site destroyed during cloning:		No
Cloning site 3':		BamHI
Site destroyed during cloning:		No
5' Sequencing primer:		cggactcagatctcgagctcaa (List of Sequencing Primers)
3' Sequencing primer:		cgctgaacttgtggccgttt
Bacteria resistance:		Kanamycin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Gentamicin
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		Michael Lerman, NCI-Frederick
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Lucy M. Anderson
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

65 - 352

CMV_immearly_promoter

10 - 562

CMV_fwd_primer

519 - 539

CMV_promoter

520 - 589

EGFP_N_primer

1279 - 1258

EGFP

1213 - 1929

ORF frame 1

1213 - 1932

EGFP_C_primer

1866 - 1887

EBV_rev_primer

2146 - 2165

f1_origin

2621 - 2315

AmpR_promoter

2700 - 2728

pBABE_3_primer

2814 - 2794

SV40_enhancer

3015 - 2800

SV40_promoter

2812 - 3080

SV40_origin

2979 - 3056

SV40pro_F_primer

3041 - 3060

ORF frame 1

3163 - 3957

NeoR/KanR

3166 - 3954

TK_PA_terminator

4132 - 4401

pBR322_origin

4549 - 5168

Unique restriction sites

AseI		7
NdeI		234
NheI		591
BglII		609
SacI		620
HindIII		622
SacII		711
BamHI		1194
AgeI		1200
XbaI		1945
AflII		2173
StuI		3112
ClaI		3131
NarI		3291
MscI		3373
FspI		3393
BstBI		3973
ApaLI		4895

Article: The von Hippel-Lindau tumor suppressor targets to mitochondria. Shiao YH et al. (Cancer Res. 2000 Jun 1. 60(11):2816-9. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13389" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.