Addgene - pEGFP-C1-wtVHL Plasmid Data

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NM_052801

NCBI: Vhl

Vhl antibodies

This is commonly
requested with

pEGFP-N1-wtVHL

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Plasmid 13390: pEGFP-C1-wtVHL

Gene/insert name:		von Hippel-Lindau
Alternative names:		VHL
Insert size (bp):		572
GenBank/Entrez ID of insert:		NM_052801
Gene/insert aliases:		Vhl, Vhlh
Species of gene(s):		R. norvegicus (rat)
Fusion proteins or tags:		GFP
Terminal:		N terminal on backbone
Vector backbone:		pEGFP-C1 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression
Backbone size (bp):		4693
Cloning site 5':		HindIII
Site destroyed during cloning:		No
Cloning site 3':		BamHI
Site destroyed during cloning:		No
5' Sequencing primer:		tctcgagctcaagcttcgg (List of Sequencing Primers)
3' Sequencing primer:		tgtggtatggctgattatgatcag tt
Bacteria resistance:		Kanamycin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Gentamicin
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		Michael Lerman, NCI-Frederick
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Lucy M. Anderson
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

65 - 352

CMV_immearly_promoter

10 - 562

CMV_fwd_primer

519 - 539

CMV_promoter

520 - 589

EGFP_N_primer

679 - 658

EGFP

613 - 1329

ORF frame 1

613 - 1923

EGFP_C_primer

1266 - 1287

SV40_PA_terminator

1946 - 2174

EBV_rev_primer

2144 - 2163

f1_origin

2619 - 2313

AmpR_promoter

2698 - 2726

pBABE_3_primer

2812 - 2792

SV40_enhancer

3013 - 2798

SV40_promoter

2810 - 3078

SV40_origin

2977 - 3054

SV40pro_F_primer

3039 - 3058

ORF frame 2

3161 - 3955

NeoR/KanR

3164 - 3952

TK_PA_terminator

4130 - 4399

pBR322_origin

4547 - 5166

Unique restriction sites

AseI		7
NdeI		234
NheI		591
AgeI		600
BglII		1339
SacI		1350
HindIII		1352
NotI		1435
SacII		1441
BamHI		1924
XbaI		1936
BclI		1946
StuI		3110
ClaI		3129
NarI		3289
MscI		3371
FspI		3391
BstBI		3971
ApaLI		4893

Article: The von Hippel-Lindau tumor suppressor targets to mitochondria. Shiao YH et al. (Cancer Res. 2000 Jun 1. 60(11):2816-9. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13390" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.