Addgene - pcDNA3 CB1 Plasmid Data

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Plasmid 13391: pcDNA3 CB1

Gene/insert name:		CB1
Insert size (bp):		1400
Gene/insert aliases:		Cnr1, CB1, CB1R
Species of gene(s):		M. musculus (mouse)
Vector backbone:		pcDNA3 (Search Vector Database)
Backbone manufacturer:		Invitrogen
Type of vector:		Mammalian expression
Backbone size (bp):		5400
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
3' Sequencing primer:		SP6
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Mary Abood
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Isolation and expression of a mouse CB1 cannabinoid receptor gene. Comparison of binding properties with those of native CB1 receptors in mouse brain and N18TG2 neuroblastoma cells. Abood ME et al. (Biochem Pharmacol. 1997 Jan 24. 53(2):207-14. Pubmed)

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