Addgene - PGKneoF2L2DTA Plasmid Data

Browse > Philippe Soriano > Hoch et al > PGKneoF2L2DTA

Price: $65.00

Plasmid Links

Related Plasmids

Philippe Soriano Lab Plasmids

This is commonly
requested with

pBS594 promoterless EGFPcre

Print Friendly Email

Plasmid 13445: PGKneoF2L2DTA

Gene/insert name:		PGKneoF2L2DTA
Alternative names:		PGKneobpA
		PGKdtabpA
Insert size (bp):		3600
Species of gene(s):		Other
Vector backbone:		na (Search Vector Database)
Type of vector:		Mammalian expression,Cre/Lox
Backbone size (bp):		2900
Cloning site 5':		See map
Site destroyed during cloning:		No
Cloning site 3':		See map
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Philippe Soriano
Terms and Licenses:		MTA

Comments: This vector contains PGKneobpA flanked by both loxP and FRT sites and PGKdtabpA.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

f1_origin

21 - 327

lacZ_a

614 - 471

M13_pUC_fwd_primer

585 - 607

M13_forward20_primer

600 - 616

T7_promoter

626 - 644

pBluescriptSK_primer

677 - 693

loxP

740 - 707

FRT

843 - 796

MSCV_rev_primer

914 - 891

mPGK_F_primer

1288 - 1307

NeoR/KanR

1369 - 2169

ORF frame 1

1369 - 2172

BGH_rev_primer

2225 - 2208

bGH_PA_terminator

2211 - 2438

FRT

2522 - 2475

loxP

2597 - 2564

MSCV_rev_primer

2735 - 2712

mPGK_F_primer

3109 - 3128

ORF frame 2

2957 - 3850

SV40_int

3851 - 3866

SV40_3_splice

3872 - 3919

BGH_rev_primer

4002 - 3985

bGH_PA_terminator

3988 - 4215

T3_promoter

4295 - 4276

M13_reverse_primer

4330 - 4312

M13_pUC_rev_primer

4351 - 4329

lac_promoter

4394 - 4365

pBR322_origin

5322 - 4703

ORF frame 2

6337 - 5477

Ampicillin

6337 - 5477

AmpR_promoter

6407 - 6379

Unique restriction sites

SacII		664
NotI		670
SmaI		775
NheI		2622
SalI		2640
HindIII		2655
EcoRV		2663
XhoI		4244
ApaI		4257
KpnI		4263

Article: Context-specific requirements for Fgfr1 signaling through Frs2 and Frs3 during mouse development. Hoch RV et al. (Development. 2006 Feb . 133(4):663-73. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13445" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.