Addgene - integrin beta6 EGFP-N3 Plasmid Data

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NM_000888

NCBI: ITGB6

This is commonly
requested with

integrin alpha9 EGFP-N3

Alpha 5 integrin- GFP

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Plasmid 13593: integrin beta6 EGFP-N3

Gene/insert name:		integrin beta 6
Alternative names:		ITGB6
Insert size (bp):		2400
GenBank/Entrez ID of insert:		NM_000888
Gene/insert aliases:		ITGB6
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		GFP
Terminal:		C terminal on backbone
Vector backbone:		EGFP-N3 (Search Vector Database)
Backbone manufacturer:		BD Biosciences
Type of vector:		Mammalian expression
Backbone size (bp):		4700
Cloning site 5':		XhoI
Site destroyed during cloning:		No
Cloning site 3':		SacII
Site destroyed during cloning:		No
5' Sequencing primer:		CMV Forward primer (List of Sequencing Primers)
3' Sequencing primer:		EGFP-N
Bacteria resistance:		Kanamycin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Dean Sheppard
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

65 - 352

CMV_immearly_promoter

10 - 562

CMV_fwd_primer

519 - 539

CMV_promoter

520 - 589

ORF frame 2

653 - 3778

ORF frame 2

atggggattgaactgctttgcctgttctttctatttctaggaaggaatgatcacgtacaa
ggtggctgtgccctgggaggtgcagaaacctgtgaagactgcctgcttattggacctcag
tgtgcctggtgtgctcaggagaattttactcatccatctggagttggcgaaaggtgtgat
accccagcaaaccttttagctaaaggatgtcaattaaacttcatcgaaaaccctgtctcc
caagtagaaatacttaaaaataagcctctcagtgtaggcagacagaaaaatagttctgac
attgttcagattgcgcctcaaagcttgatccttaagttgagaccaggtggtgcgcagact
ctgcaggtgcatgtccgccagactgaggactacccggtggatttgtattacctcatggac
ctctccgcctccatggatgacgacctcaacacaataaaggagctgggctcccggctttcc
aaagagatgtctaaattaaccagcaactttagactgggcttcggatcttttgtggaaaaa
cctgtatcccctttcgtgaaaacaacaccagaagaaattgccaacccttgcagtagtatt
ccatacttctgtttacctacatttggattcaagcacattttgccattgacaaatgatgct
gaaagattcaatgaaattgtgaagaatcagaaaatttctgctaatattgacacacccgaa
ggtggatttgatgcaattatgcaagctgctgtgtgtaaggaaaaaattggctggcggaat
gactccctccacctcctggtctttgtgagtgatgctgattctcattttggaatggacagc
aaactagcaggcatcgtcattcctaatgacgggctctgtcacttggacagcaagaatgaa
tactccatgtcaactgtcttggaatatccaacaattggacaactcattgataaactggta
caaaacaacgtgttattgatcttcgctgtaacccaagaacaagttcatttatatgagaat
tacgcaaaacttattcctggagctacagtaggtctacttcagaaggactccggaaacatt
ctccagctgatcatctcagcttatgaagaactgcggtctgaggtggaactggaagtatta
ggagacactgaaggactcaacttgtcatttacagccatctgtaacaacggtaccctcttc
caacaccaaaagaaatgctctcacatgaaagtgggagacacagcttccttcagcgtgact
gtgaatatcccacactgcgagagaagaagcaggcacattatcataaagcctgtggggctg
ggggatgccctggaattacttgtcagcccagaatgcaactgcgactgtcagaaagaagtg
gaagtgaacagctccaaatgtcaccacgggaacggctctttccagtgtggggtgtgtgcc
tgccaccctggccacatggggcctcgctgtgagtgtggcgaggacatgctgagcacagat
tcctgcaaggaggccccagatcatccctcctgcagcggaaggggtgactgctactgtggg
cagtgtatctgccacttgtctccctatggaaacatttatgggccttattgccagtgtgac
aatttctcctgcgtgagacacaaagggctgctctgcggaggtaacggcgactgtgactgt
ggtgaatgtgtgtgcaggagcggctggactggcgagtactgcaactgcaccaccagcacg
gactcctgcgtctctgaagatggagtgctctgcagcgggcgcggggactgtgtttgtggc
aagtgtgtttgcacaaaccctggagcctcaggaccaacctgtgaacgatgtcctacctgt
ggtgacccctgtaactctaaacggagctgcattgagtgccacctgtcagcagctggccaa
gcccgagaagaatgtgtggacaagtgcaaactagctggtgcgaccatcagtgaagaagaa
gatttctcaaaggatggttctgtttcctgctctctgcaaggagaaaatgaatgtcttatt
acattcctaataactacagataatgaggggaaaaccatcattcacagcatcaatgaaaaa
gattgtccgaagcctccaaacattcccatgatcatgttaggggtttccctggctattctt
ctcatcggggttgtcctactgtgcatctggaagctactggtgtcatttcatgatcgtaaa
gaagttgccaaatttgaagcagaacgatcaaaagccaagtggcaaacgggaaccaatcca
ctctacagaggatccacaagtacttttaaaaatgtaacttataaacacagggaaaaacaa
aaggtagacctttccacagattgcTCTAGAGCGGCCGCCACCGCGGgcccgggatccatc
gccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgag
ctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcc
acctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctgg
cccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccac
atgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacc
atcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgac
accctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag
aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcag
ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgac
aaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcac
atggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac
aagtaa

EGFP_N_primer

3125 - 3104

EGFP

3059 - 3775

EGFP_C_primer

3712 - 3733

EBV_rev_primer

3992 - 4011

f1_origin

4467 - 4161

AmpR_promoter

4546 - 4574

pBABE_3_primer

4660 - 4640

SV40_enhancer

4861 - 4646

SV40_promoter

4658 - 4926

SV40_origin

4825 - 4902

SV40pro_F_primer

4887 - 4906

ORF frame 2

5009 - 5803

NeoR/KanR

5012 - 5800

TK_PA_terminator

5978 - 6247

pBR322_origin

6395 - 7014

Unique restriction sites

AseI		7
NdeI		234
NheI		591
BglII		609
XhoI		613
HindIII		973
KpnI		1845
SacII		3036
ApaI		3041
SmaI		3042
HpaI		3900
StuI		4958
ClaI		4977
NarI		5137
BstBI		5819
ApaLI		6741

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13593" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.