Addgene - pRho-Cre Plasmid Data

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Plasmid 13779: pRho-Cre

Gene/insert name:		Rhodopsin promoter-Cre
Alternative names:		Cre recombinase
Insert size (bp):		3393
Fusion proteins or tags:		Myc
Terminal:		C terminal on insert
Vector backbone:		pCAGEN? (Search Vector Database)
Type of vector:		Mammalian expression,Cre/Lox
Backbone size (bp):		5362
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		NotI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		The bovine rhodopsin promoter was from -2174-lacZ (Zack et al., Neuron 6, 187-199 (1991)) obtained from Dr. D. Zack (Johns Hopkins University). The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template.
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Connie Cepko
Terms and Licenses:		MTA

Comments: The rhodopsin promoter-Cre is specific for photorecetor cells in the retina. Cre has a Myc-tag at its C-terminus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CRE_NLS

2332 - 3327

ORF frame 1

2320 - 3390

rb_glob_PA_terminator

3410 - 3911

M13_reverse_primer

3971 - 3953

M13_pUC_rev_primer

3992 - 3970

lac_promoter

4035 - 4006

SV40_origin

4176 - 4253

SV40_promoter

4141 - 4320

SV40pro_F_primer

4238 - 4257

SV40_PA_terminator

4334 - 4465

EBV_rev_primer

4422 - 4441

pBR322_origin

5308 - 4689

ORF frame 3

6323 - 5463

Ampicillin

6323 - 5463

AmpR_promoter

6393 - 6365

Unique restriction sites

SalI		1
PmlI		395
XhoI		578
XbaI		2280
EcoRI		2302
NruI		2534
EcoRV		2871
ClaI		3126
NotI		3394
EagI		3394
FspI		5758

Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13779" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.