Addgene - pNrl-Cre Plasmid Data

Gene/insert name:		Nrl promoter-Cre
Alternative names:		Nrl
Insert size (bp):		4308
Gene/insert aliases:		Nrl, AW492574, D14H14S46E
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		Cre
Terminal:		C terminal on backbone
Vector backbone:		na (Search Vector Database)
Type of vector:		Mammalian expression,Cre/Lox
Backbone size (bp):		3100
Cloning site 5':		SalI
Site destroyed during cloning:		No
Cloning site 3':		NotI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template.
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Connie Cepko
Terms and Licenses:		MTA

Comments: Mouse Nrl promoter was isolated by genomic PCR using B6 mouse genomic DNA as a template. The Nrl promoter-Cre is specific for rod photorecetor cells in the retina. Cre has a Myc-tag at its C-terminus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CRE_NLS

3247 - 4242

ORF frame 1

3235 - 4305

rb_glob_PA_terminator

4325 - 4826

M13_reverse_primer

4886 - 4868

M13_pUC_rev_primer

4907 - 4885

lac_promoter

4950 - 4921

SV40_origin

5091 - 5168

SV40_promoter

5056 - 5235

SV40pro_F_primer

5153 - 5172

SV40_PA_terminator

5249 - 5380

EBV_rev_primer

5337 - 5356

pBR322_origin

6223 - 5604

ORF frame 3

7238 - 6378

Ampicillin

7238 - 6378

AmpR_promoter

7308 - 7280

Unique restriction sites

SalI		1
XbaI		31
SmaI		186
SacI		227
ApaI		1158
AflII		2128
XhoI		3035
EcoRI		3217
NruI		3449
ClaI		4041
NotI		4309
EagI		4309
PstI		4849
FspI		6673

Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13780" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.