Addgene - pSLAX13 Flag (CT#231) Plasmid Data

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Plasmid 14026: pSLAX13 Flag (CT#231)

Gene/insert name:		None
Insert size (bp):		Unknown
Fusion proteins or tags:		Flag
Terminal:		Unknown
Vector backbone:		pSLAX13 (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		3000
5' Sequencing primer:		T3 (List of Sequencing Primers)
3' Sequencing primer:		T7
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Principal Investigator:		Cliff Tabin
Terms and Licenses:		MTA

Comments: pSlax13 is a convenient entry vector for cloning into the pRCAS BP vectors. Clone your gene into pSlax13, then cut with ClaI to move your gene into pRCAS BP. pSlax13 has the advantage of allowing all the cloning steps to be carried out in a relatively small, high copy number vector.

pSlax13 was constructed by placing a ClaI adapter fragment into a Bluescript derivative. See author's map for information on the multiple cloning site.

For more information on the RCAS system, visit http://home.ncifcrf.gov/hivdrp/RCAS/index.html .

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene misexpression in chick limb buds using avian replication-competent retroviruses. Logan M et al. (Methods. 1998 Apr . 14(4):407-20. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 14026" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.