Addgene - pMSCV neo WT Akt1 Plasmid Data

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Plasmid 14549: pMSCV neo WT Akt1

Gene/insert name:		Akt1
Insert size (bp):		Unknown
Gene/insert aliases:		Akt1, Akt, PKB, Rac, PKB/Akt, PKBalpha
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		HA tag
Terminal:		Unknown
Vector backbone:		pMSCV neo (Search Vector Database)
Backbone manufacturer:		BD Biosciences
Type of vector:		Mammalian expression,Retroviral
Backbone size (bp):		6500
5' Sequencing primer:		MSCV (List of Sequencing Primers)
3' Sequencing primer:		MSCV rev
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		Philip Tsichlis
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Joan Brugge
Terms and Licenses:		MTA

Comments: wild-type Akt1

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial-mesenchymal transition. Irie HY et al. (J Cell Biol. 2005 Dec 19. 171(6):1023-34. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 14549" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.