Addgene - pPROK AdoHcyase Plasmid Data

Browse > Michael Hershfield > Coulter-Karis et al > pPROK AdoHcyase

Price: $65.00

Plasmid Links

Related Plasmids

Michael Hershfield Lab Plasmids

Other Links

NCBI: AHCY

Print Friendly Email

Plasmid 14683: pPROK AdoHcyase

Gene/insert name:		adenosyl-homocysteine hydrolase
Alternative names:		AdoHcyase
		AHCY
Insert size (bp):		1300
Gene/insert aliases:		AHCY, SAHH
Species of gene(s):		H. sapiens (human)
Vector backbone:		pPROK-1 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Bacterial expression
Backbone size (bp):		4600
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		tac promoter (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Michael Hershfield
Terms and Licenses:		MTA

Comments: The AdoHcyase coding sequence was derived from human placenta AdoHcyase cDNA clone 20-1.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Article: Sequence of full length cDNA for human S-adenosylhomocysteine hydrolase. Coulter-Karis DE et al. (Ann Hum Genet. 1989 May . 53(Pt 2):169-75. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 14683" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.