OMP-SpH-ACNF TV
(Plasmid #15513)

• Depositing Lab: Peter Mombaerts
• Publication: Bozza et al Neuron. 2004 Apr 8. 42(1):9-21.

Backbone

• Vector backbone: pGEM5
• Backbone size w/o insert (bp): 3000
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: olfactory marker protein
• Alt name: OMP
• Alt name: Omp
• Species: M. musculus (mouse)
• Mutation: OMP coding region and 3' end were replaced by synaptopHluorin in this targeting vector.
• GenBank ID: BC132327
• Entrez Gene: Omp

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ? (unknown if destroyed)
• 3′ cloning site: ? (unknown if destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: synaptopHluorin was obtained from Dr. James Rothman

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Targeting vector can be linearized with XhoI.

The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/004946.html