Addgene - pRK5-HA-Ubiquitin-KO Plasmid Data

Gene/insert name:		Ubiquitin C
Alternative names:		Ub
Insert size (bp):		220
Gene/insert aliases:		UBC, HMG20
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		KO: all lysines mutated to arginines
Fusion proteins or tags:		HA
Terminal:		N terminal on backbone
Vector backbone:		pRK5-HA (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4800
Cloning site 5':		SalI
Site destroyed during cloning:		No
Cloning site 3':		NotI
Site destroyed during cloning:		No
5' Sequencing primer:		SP6 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Ted Dawson
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Parkin mediates nonclassical, proteasomal-independent ubiquitination of synphilin-1: implications for Lewy body formation. Lim KL et al. (J Neurosci. 2005 Feb 23. 25(8):2002-9. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 17603" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.