Addgene - pBABE-hygro Plasmid Data

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This is commonly
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pBABE-neo

pBABE-puro

pBABE-zeo ( pBABE-bleo)

pBABE-hygro- hTERT

pBABE GFP

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Plasmid 1765: pBABE-hygro

Gene/insert name:		none
Insert size (bp):		Unknown
Vector backbone:		pBABE-hygro (Search Vector Database)
Type of vector:		Mammalian expression,Retroviral
Backbone size (bp):		5558
5' Sequencing primer:		pBABE 5' (List of Sequencing Primers)
3' Sequencing primer:		pBABE 3'
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Hygromycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Bob Weinberg
Additional Documents:		pBABE protocol (PDF)
Terms and Licenses:		MTA

Comments: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that Addgene supplies pCL-Eco (#12371), VSV-G (#8454), and a gag/pol expression vector (#8455).

Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

AmpR_promoter

32 - 4

5_LTR2

102 - 569

3MoMuLV_LTR

108 - 569

psi_plus_pack

639 - 1440

pBABE_5_primer

1408 - 1424

pBABE_3_primer

1518 - 1498

SV40_promoter

1516 - 1784

SV40_enhancer

1883 - 1504

SV40_origin

1683 - 1760

SV40_promoter

1636 - 1838

SV40pro_F_primer

1745 - 1764

ORF frame 1

1906 - 2931

hygroB

1921 - 2928

pBR322_origin

2979 - 3504

3MoMuLV_LTR

3604 - 4191

5_LTR2

3646 - 4185

Ampicillin

5540 - 4680

Unique restriction sites

SpeI		706
BamHI		1445
EcoRI		1469
SalI		1487
StuI		1816
HindIII		1833
BglII		1879
NdeI		2356
SacII		2686
NheI		3634
FspI		4975

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1765" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.