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Plasmid 1805: Phleo/Xpress

Gene/insert name:		Phleo/Xpress
Insert size (bp):		922
Fusion proteins or tags:		Xpress
Terminal:		C terminal on insert
Vector backbone:		pBluescript II KS (Search Vector Database)
Backbone manufacturer:		Stratagene
Type of vector:		Bacterial expression
Backbone size (bp):		3000
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Phleomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Christian Tschudi
Terms and Licenses:		MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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Article: In vivo epitope tagging of Trypanosoma brucei genes using a one step PCR-based strategy. Shen S et al. (Mol Biochem Parasitol 2001 Mar;113(1):171-3. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1805" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.