Addgene - pRET.IL.IRES-EGFP (No HSV-TK) Plasmid Data

Browse > Philip Leder > Ishida et al > pRET.IL.IRES-EGFP (No HSV-TK)

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Plasmid 1836: pRET.IL.IRES-EGFP (No HSV-TK)

Gene/insert name:		None
Alternative names:		retroviral RET construct
		removable exon trap
		Type I vector
Insert size (bp):		Unknown
Vector backbone:		pRET (Search Vector Database)
Type of vector:		Mammalian expression,Retroviral,Cre/Lox
Backbone size (bp):		Unknown
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Philip Leder
Terms and Licenses:		MTA

Comments: See scanned map (#4). The HSV-TK cassette is eliminated from this vector. Virus production should be fine, but you can not titrate the virus accurately because of the lack of the HSV-TK cassette, the only constitutive marker in RET vectors. You can easily excise all the essential components as an XbaI-XbaI fragment. To obtain larger numbers of G418-resistant clones, this vector carries a strong NEO promoter. This RET vector would be the first choice for making gene-disrupted mice by using transfected or infected ES cells. (Leder #C-1023)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida Y et al. (Nucleic Acids Res 1999 Dec 15;27(24):e35. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1836" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.