Addgene - pRET.IL.PGK-TK Plasmid Data

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Plasmid 1837: pRET.IL.PGK-TK

Gene/insert name:		None
Alternative names:		retroviral RET construct
		removable exon trap
		Type I vector
Insert size (bp):		Unknown
Vector backbone:		pRET (Search Vector Database)
Type of vector:		Mammalian expression,Retroviral,Cre/Lox
Backbone size (bp):		Unknown
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Philip Leder
Terms and Licenses:		MTA

Comments: See scanned map (#5). This is a modified RET vector exclusively for the DEL.BANK project (JC Schimenti, the Jackson Lab): the PGK promoter for the HSV-TK gene is much stronger than the MC1 promoter in other RET vectors. Virus production is virtually impossible. Either the PGK promoter or PGK poly A signal seems to be very inhibitory for the transcription of the virus genome. You can easily excise the essential components as an XbaI-XbaI fragment. (Leder #C-1024)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida Y et al. (Nucleic Acids Res 1999 Dec 15;27(24):e35. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1837" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.