Addgene - pRET.IIS.IRES-EGFP Plasmid Data

Browse > Philip Leder > Ishida et al > pRET.IIS.IRES-EGFP

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Plasmid 1838: pRET.IIS.IRES-EGFP

Gene/insert name:		None
Alternative names:		retroviral RET construct
		removable exon trap
		Type II vector
Insert size (bp):		Unknown
Vector backbone:		pRET (Search Vector Database)
Type of vector:		Mammalian expression,Retroviral,Cre/Lox
Backbone size (bp):		Unknown
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Philip Leder
Terms and Licenses:		MTA

Comments: See scanned map (#3). This is a modified RET-EGFP vector for reasonably high-titer virus production, up to ~4x10^5 cfu/ml. However, the selectivity for the intragenic integration would not be as high as that achieved by other RET vectors (Type I). An additional XbaI site between EGFP and GHpA prevents isolation of essential elements of the vector on a single XbaI-XbaI fragment; this vector is not suitable for transfection. (Leder #C-1022)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida Y et al. (Nucleic Acids Res 1999 Dec 15;27(24):e35. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1838" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.