Addgene - CMV-Brainbow-1.1 M Plasmid Data

Gene/insert name:		Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
Insert size (bp):		Unknown
GenBank/Entrez ID of insert:		AB128820
Species of gene(s):		Other
Vector backbone:		pEGFP-N1 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression,Cre/Lox
Backbone size (bp):		Unknown
5' Sequencing primer:		CMV-F (List of Sequencing Primers)
Bacteria resistance:		Kanamycin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Joshua Sanes
Additional Documents:		Brainbow Plasmids Information (PDF)
Terms and Licenses:		MTA, Clontech LULL

Comments: The Author's Map is a representative Brainbow 1.1 construct (M= membrane tethered).

Please see attached PDF for important additional information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

65 - 352

CMV_immearly_promoter

10 - 562

CMV_fwd_primer

519 - 539

CMV_promoter

520 - 589

loxP

635 - 602

loxP

602 - 635

loxP

676 - 643

loxP

643 - 676

loxP

686 - 719

ORF frame 1

739 - 1395

EBV_rev_primer

1609 - 1628

loxP

1670 - 1637

loxP

1637 - 1670

ORF frame 3

1686 - 2507

EBV_rev_primer

2726 - 2745

loxP

2872 - 2839

loxP

2839 - 2872

EGFP_N_primer

3063 - 3042

ORF frame 3

2892 - 3716

EYFP

2997 - 3704

EGFP_C_primer

3650 - 3671

EBV_rev_primer

3930 - 3949

loxP

3975 - 4008

EGFP_N_primer

4203 - 4182

ORF frame 3

4032 - 4856

ECFP

4137 - 4853

EGFP_C_primer

4790 - 4811

EBV_rev_primer

5047 - 5066

f1_origin

5522 - 5216

AmpR_promoter

5601 - 5629

pBABE_3_primer

5715 - 5695

SV40_enhancer

5916 - 5701

SV40_promoter

5713 - 5981

SV40_origin

5880 - 5957

SV40pro_F_primer

5942 - 5961

ORF frame 1

6064 - 6858

NeoR/KanR

6067 - 6855

TK_PA_terminator

7033 - 7302

pBR322_origin

7450 - 8069

Unique restriction sites

NdeI		234
NheI		591
SacI		641
BglII		726
PmlI		2511
BclI		2873
ClaI		6032

Article: Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Livet J et al. (Nature. 2007 Nov 1. 450(7166):56-62. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 18722" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.