Addgene - p70 S6 kinase Plasmid Data

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NCBI: Rps6kb1

Rps6kb1 antibodies

This is commonly
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Raf-1 GST RBD 1-149

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Plasmid 1892: p70 S6 kinase

Gene/insert name:		p70 alpha1 S6 kinase
Alternative names:		p70 S6 kinase
Insert size (bp):		1800
Gene/insert aliases:		Rps6kb1
Species of gene(s):		R. norvegicus (rat)
Fusion proteins or tags:		HA
Terminal:		N terminal on insert
Vector backbone:		PMT2 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		5100
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Joseph Avruch
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

SV40pro_F_primer

102 - 83

SV40_promoter

211 - 9

SV40_origin

164 - 87

SV40_promoter

331 - 63

SV40_enhancer

134 - 349

pBABE_3_primer

329 - 349

ORF frame 2

1315 - 332

synth_int

650 - 1065

1125 - 1099

1099 - 1125

ORF frame 1

1096 - 2709

SV40_PA_terminator

3743 - 3874

EBV_rev_primer

3831 - 3850

pBR322_origin

5179 - 4571

Ampicillin

6195 - 5334

AmpR_promoter

6265 - 6237

pGEX_3_primer

6447 - 6425

lacZ_a

6789 - 6646

M13_pUC_fwd_primer

6760 - 6782

M13_forward20_primer

6775 - 6791

Unique restriction sites

StuI		30
MscI		995
PstI		1068
NotI		1085
ApaI		1130
SpeI		1360
AgeI		1621
XhoI		2090
AflII		3278
HpaI		3739
EcoRV		3983
BclI		4076
ClaI		4082
BstBI		4179
NheI		4307
NarI		6632

Article: Multiple independent inputs are required for activation of the p70 S6 kinase. Weng QP et al. (Mol Cell Biol 1995 May;15(5):2333-40. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1892" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.