Addgene - p70 delta2-46 delta CT104 Plasmid Data

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Plasmid 1898: p70 delta2-46 delta CT104

Gene/insert name:		p70 alpha1 S6 kinase
Alternative names:		p70 S6 kinase
Insert size (bp):		1300
Gene/insert aliases:		Rps6kb1
Species of gene(s):		R. norvegicus (rat)
Relevant mutations/deletions:		delta2-46, deltaCT104 (deletion of aa 2-46 and amino acids after residue 421)
Fusion proteins or tags:		HA
Terminal:		N terminal on insert
Vector backbone:		PMT2 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		5100
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Plasmid Provided In:		DH5a
Principal Investigator:		Joseph Avruch
Terms and Licenses:		MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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Article: Multiple independent inputs are required for activation of the p70 S6 kinase. Weng QP et al. (Mol Cell Biol 1995 May;15(5):2333-40. Pubmed)

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