Addgene - GST-14-3-3wt (pEBG) Plasmid Data

Gene/insert name:		14-3-3 zeta
Alternative names:		14.3.3
		Ywhaz
Insert size (bp):		Unknown
Gene/insert aliases:		Ywhaz, AI596267, AL022924, AU020854, 1110013I11Rik
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		GST
Terminal:		N terminal on backbone
Vector backbone:		pEBG (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		6100
5' Sequencing primer:		pGEX5' (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Joseph Avruch
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: A dimeric 14-3-3 protein is an essential cofactor for Raf kinase activity. Tzivion G et al. (Nature 1998 Jul 2;394(6688):88-92. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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