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Browse > Joseph Avruch > Tzivion et al > GST-14-3-3dm (pEBG) Price: $65.00 Plasmid Links Related Plasmids Other Links This is commonly
requested with Plasmid 1943: GST-14-3-3dm (pEBG)
| Gene/insert name: | | 14-3-3 zeta | |
| Alternative names: | | 14.3.3 | |
| | Ywhaz | |
| Insert size (bp): | | Unknown | |
| Gene/insert aliases: | | Ywhaz, AI596267, AL022924, AU020854, 1110013I11Rik | |
| Species of gene(s): | | M. musculus (mouse)
| |
| Relevant mutations/deletions: | | Dimerization-deficient mutant of 14-3-3zeta aa: E5K, L12AE to Q12QR, Y82Q, K85N, and E87Q | |
| Fusion proteins or tags: | | GST | |
| Terminal: | | N terminal on backbone | |
| Vector backbone: | | pEBG
(Search Vector Database) | |
| Type of vector: | | Mammalian expression | |
| Backbone size (bp): | | 6100 | |
| Cloning site 5': | | BamHI | |
| Site destroyed during cloning: | | Unknown | |
| Cloning site 3': | | EcoRV | |
| Site destroyed during cloning: | | Unknown | |
| 5' Sequencing primer: | | CMV-F (List of Sequencing Primers) | |
| Bacteria resistance: | | Ampicillin | |
| High or low copy: | | High Copy | |
| Grow in standard E. coli @ 37C: | | Yes | |
| Sequence: | | View sequence | | Plasmid Provided In: | | DH5a | | Principal Investigator: | | Joseph Avruch | | Terms and Licenses: | | MTA | Click on map to enlargeArticle: A dimeric 14-3-3 protein is an essential cofactor for Raf kinase activity. Tzivion G et al. (Nature 1998 Jul 2;394(6688):88-92. Pubmed) Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1943" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.
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