Addgene - GST-14-3-3wt (pGEX) Plasmid Data

Gene/insert name:		14-3-3 zeta
Alternative names:		14.3.3
		Ywhaz
Insert size (bp):		Unknown
Gene/insert aliases:		Ywhaz, AI596267, AL022924, AU020854, 1110013I11Rik
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		GST
Terminal:		N terminal on backbone
Vector backbone:		pGEX-KG (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		5000
5' Sequencing primer:		pGEX5' (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Joseph Avruch
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Identification of the 14.3.3 zeta domains important for self-association and Raf binding. Luo ZJ et al. (J Biol Chem 1995 Oct 6;270(40):23681-7. Pubmed)

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