Addgene - FLAG-MST1(K59R) Plasmid Data

Browse > Joseph Avruch > Lin et al > FLAG-MST1(K59R)

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NCBI: Mst1

This is commonly
requested with

FLAG-MST1

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Plasmid 1966: FLAG-MST1(K59R)

Gene/insert name:		MST1
Insert size (bp):		Unknown
Gene/insert aliases:		Mst1, Hgfl, D3F15S2h, DNF15S2h, D9H3F15S2
Species of gene(s):		M. musculus (mouse)
Relevant mutations/deletions:		Kinase inactive: K59R, also caspase 3 cleavage site mutated: D326N
Fusion proteins or tags:		FLAG
Terminal:		Unknown
Vector backbone:		pCMV5 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4657
5' Sequencing primer:		CMV Forward (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		cDNA from Leonard Zon (Children's Hospital, Boston)
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Joseph Avruch
Terms and Licenses:		MTA

Comments: Kinase inactive due to mutation at the ATP binding site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Death-associated protein 4 binds MST1 and augments MST1-induced apoptosis. Lin Y et al. (J Biol Chem 2002 Dec 13;277(50):47991-8001. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1966" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.