Addgene - ChR2-2A-Halo Plasmid Data

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Plasmid 22047: ChR2-2A-Halo

Gene/insert name:		ChR2.GFP-2A-Halo.YFP
Alternative names:		NpHR
		channelrhodopsin
		halorhodopsin
Insert size (bp):		3356
Species of gene(s):		C. reinhardtii N. pharaonis
Fusion proteins or tags:		GFP and YFP
Terminal:		C terminal on insert
Vector backbone:		pEGFP-N1 (Search Vector Database)
Backbone manufacturer:		clontech
Type of vector:		Mammalian expression
Backbone size (bp):		3963
Cloning site 5':		Kpn1
Site destroyed during cloning:		Unknown
Cloning site 3':		EcoRI
Site destroyed during cloning:		Unknown
5' Sequencing primer:		AGCAGAGCTGGTTTAGTGAA (List of Sequencing Primers)
3' Sequencing primer:		TAAAACCTCTACAAATGTGG
Bacteria resistance:		Kanamycin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

65 - 352

CMV_immearly_promoter

10 - 562

CMV_fwd_primer

519 - 539

CMV_promoter

520 - 589

EGFP_N_primer

1625 - 1604

EGFP

1571 - 2275

ORF frame 2

2884 - 1076

EGFP_C_primer

2212 - 2233

ORF frame 2

608 - 3970

ORF frame 2

atgggtaccgactatggcggcgctttgtctgccgtcggacgcgaacttttgttcgttact
aatcctgtggtggtgaacgggtccgtcctggtccctgaggatcaatgttactgtgccgga
tggattgaatctcgcggcacgaacggcgctcagaccgcgtcaaatgtcctgcagtggctt
gcagcaggattcagcattttgctgctgatgttctatgcctaccaaacctggaaatctaca
tgcggctgggaggagatctatgtgtgcgccattgaaatggttaaggtgattctcgagttc
ttttttgagtttaagaatccctctatgctctaccttgccacaggacaccgggtgcagtgg
ctgcgctatgcagagtggctgctcacttgtcctgtcatccttatccacctgagcaacctc
accggcctgagcaacgactacagcaggagaaccatgggactccttgtctcagacatcggg
actatcgtgtggggggctaccagcgccatggcaaccggctatgttaaagtcatcttcttt
tgtcttggattgtgctatggcgcgaacacattttttcacgccgccaaagcatatatcgag
ggttatcatactgtgccaaagggtcggtgccgccaggtcgtgaccggcatggcatggctg
tttttcgtgagctggggtatgttcccaattctcttcattttggggcccgaaggttttggc
gtcctgagcgtctatggctccaccgtaggtcacacgattattgatctgatgagtaaaaat
tgttgggggttgttgggacactacctgcgcgtcctgatccacgagcacatattgattcac
ggagatatccgcaaaaccaccaaactgaacatcggcggaacggagatcgaggtcgagact
ctcgtcgaagacgaagccgaggccggagccgtgccagcggcaccggtagtagcagtgagc
aagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgta
aacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctg
accctgaagttcatttgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacc
accctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgac
ttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggac
gacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgc
atcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggag
tacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaag
gtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactac
cagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagc
acccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggag
ttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaaacagaaaatt
gtggcaccagtgaaacagactttgaattttgaccttctcaagttggcgggagacgtcgag
tccaaccctgggccctctagaactgagaccctcccacccgtgactgaaagcgccgtcgct
ctgcaagcagaggttacccagcgggagctgttcgagttcgtcctcaacgaccccctcctg
gcttctagcctctacatcaacattgctctggcaggcctgtctatactgctgttcgtcttc
atgaccaggggactcgatgaccctagggctaaactgattgcagtgagcacaattctggtt
cccgtggtctctatcgcttcctacactgggctggcatctggtctcacaatcagtgtcctg
gaaatgccagctggccactttgccgaagggagttctgtcatgctgggaggcgaagaggtc
gatggggttgtcacaatgtggggtcgctacctcacctgggctctcagtacccccatgatc
ctgctggcactcggactcctggccggaagtaacgccaccaaactcttcactgctattaca
ttcgatatcgccatgtgcgtgaccgggctcgcagctgccctcaccaccagcagccatctg
atgagatggttttggtatgccatctcttgtgcctgctttctggtggtgctgtatatcctg
ctggtggagtgggctcaggatgccaaggctgcagggacagccgacatgtttaatacactg
aagctgctcactgtggtgatgtggctgggttaccctatcgtttgggcactcggcgtggag
ggaatcgcagttctgcctgttggtgtgacaagctggggctactccttcctggacattgtg
gccaagtatatttttgcctttctgctgctgaattatctgacttccaatgagtccgtggtg
tccggctccatactggacgtgccatccgccagcggcacacctgccgatgaccgaccggta
gtagcagtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctg
gacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacc
tacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccc
accctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatg
aagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatc
ttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacacc
ctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgggg
cacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaag
aacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctc
gccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaac
cactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatg
gtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
taa

EGFP_N_primer

3317 - 3296

EYFP

3254 - 3961

EGFP_C_primer

3904 - 3925

EBV_rev_primer

4198 - 4217

f1_origin

4673 - 4367

AmpR_promoter

4752 - 4780

pBABE_3_primer

4866 - 4846

SV40_enhancer

5067 - 4852

SV40_promoter

4864 - 5132

SV40_origin

5031 - 5108

SV40pro_F_primer

5093 - 5112

ORF frame 1

5215 - 6009

NeoR/KanR

5218 - 6006

TK_PA_terminator

6184 - 6453

pBR322_origin

6601 - 7220

Unique restriction sites

AseI		7
NdeI		234
KpnI		615
BglII		861
XhoI		899
EcoRI		3971
NotI		3987
HpaI		4106
AflII		4225
ClaI		5183
NarI		5343
FspI		5445
BstBI		6025
ApaLI		6947

Article: Informational lesions: optical perturbation of spike timing and neural synchrony via microbial opsin gene fusions. Han X et al. (Front Mol Neurosci. 2009 . 2():12. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22047" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.