Addgene - LCAG-ChR2-GFP Plasmid Data

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Plasmid 22049: LCAG-ChR2-GFP

Gene/insert name:		ChR2-GFP
Alternative names:		channelrhodopsin-2
Insert size (bp):		1677
GenBank/Entrez ID of insert:		EU714030
Species of gene(s):		C. reinhardtii
Relevant mutations/deletions:		N/A
Fusion proteins or tags:		GFP
Terminal:		C terminal on insert
Vector backbone:		LB2 (Search Vector Database)
Backbone manufacturer:		Patrick Stern
Type of vector:		Mammalian expression,Lentiviral,Mouse Targeting
Backbone size (bp):		8969
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		5' ATTGATTTGATACCGCGGGC 3' (List of Sequencing Primers)
3' Sequencing primer:		5' CAAAGAGATAGCTTGGTATT 3'
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		Stbl3
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

lac_promoter

525 - 554

M13_pUC_rev_primer

568 - 590

M13_reverse_primer

589 - 607

HIV-1_5_LTR

869 - 1049

truncHIV-1_3_LTR

869 - 1049

HIV-1_psi_pack

1160 - 1204

RRE

1720 - 1953

ORF frame 2

1598 - 2485

cPPT

2484 - 2499

CAG_enhancer

3123 - 3410

ORF frame 2

4393 - 3785

pCAG_F_primer

4728 - 4747

ORF frame 1

4816 - 6480

EGFP_N_primer

5827 - 5806

EGFP

5773 - 6477

EGFP_C_primer

6414 - 6435

WPRE

6677 - 7264

ORF frame 1

6778 - 7419

pBluescriptKS_primer

7283 - 7267

cPPT

8284 - 8299

U3PPT

8284 - 8305

bGH_PA_terminator

8423 - 8638

HIV-1_5_LTR

8652 - 8832

truncHIV-1_3_LTR

8652 - 8832

pBR322_origin

9498 - 8879

ORF frame 2

10513 - 9653

Ampicillin

10513 - 9653

AmpR_promoter

10583 - 10555

Unique restriction sites

SpeI		252
XbaI		4673
SmaI		4792
BamHI		4804
XhoI		5101
AgeI		5751
EcoRI		6481
FspI		9948

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22049" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.