Addgene - Fubi-Halo-GFP Plasmid Data

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Plasmid 22050: Fubi-Halo-GFP

Gene/insert name:		Halo-GFP
Alternative names:		Halo
		halorhodopsin
		NpHR
Insert size (bp):		1617
GenBank/Entrez ID of insert:		EF537649
Species of gene(s):		N. pharaonis
Relevant mutations/deletions:		N. pharaonis halorhodopsin is mammalian codon-optimized
Fusion proteins or tags:		GFP
Terminal:		C terminal on insert
Vector backbone:		FUGW (Search Vector Database)
Backbone manufacturer:		Lois/Baltimore
Type of vector:		Mammalian expression,Lentiviral
Backbone size (bp):		9114
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		5' GCACCTTTTGAAATGTAATC 3' (List of Sequencing Primers)
3' Sequencing primer:		5' AGAATACCAGTCAATCTTTCAC 3'
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		Stbl3
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

318 - 605

CMV_immearly_promoter

239 - 815

CMV_fwd_primer

772 - 792

HIV-1_5_LTR

835 - 1015

truncHIV-1_3_LTR

835 - 1015

HIV-1_psi_pack

1126 - 1170

RRE

1680 - 1913

ORF frame 1

1558 - 2445

cPPT

2444 - 2459

hUbC_promoter

2618 - 3771

ORF frame 2

3794 - 5398

EGFP_N_primer

4745 - 4724

EGFP

4691 - 5395

EGFP_C_primer

5332 - 5353

WPRE

5425 - 6012

pBluescriptKS_primer

6031 - 6015

ORF frame 2

5513 - 6562

cPPT

6199 - 6214

U3PPT

6199 - 6220

HIV-1_5_LTR

6536 - 6716

truncHIV-1_3_LTR

6536 - 6716

BGH_rev_primer

6759 - 6742

bGH_PA_terminator

6745 - 6972

f1_origin

7035 - 7341

pBABE_3_primer

7475 - 7455

SV40_enhancer

7676 - 7461

SV40_promoter

7473 - 7741

SV40_origin

7640 - 7717

SV40pro_F_primer

7702 - 7721

EM7_promoter

7835 - 7902

bleo

7903 - 8274

sh_ble

7903 - 8277

SV40_PA_terminator

8410 - 8529

EBV_rev_primer

8498 - 8517

M13_reverse_primer

8591 - 8573

M13_pUC_rev_primer

8612 - 8590

lac_promoter

8655 - 8626

pBR322_origin

9583 - 8964

ORF frame 3

10598 - 9738

Ampicillin

10598 - 9738

AmpR_promoter

10668 - 10640

Unique restriction sites

NdeI		487
NotI		1525
BamHI		3782
AgeI		4669
EcoRI		5399
XhoI		6027
KpnI		6149
ApaI		6721
FspI		10033

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22050" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.