Addgene - Fubi-CHR2-GFP Plasmid Data

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Plasmid 22051: Fubi-CHR2-GFP

Gene/insert name:		ChR2-GFP
Alternative names:		channelrhodopsin-2
Insert size (bp):		1677
GenBank/Entrez ID of insert:		EU714030
Species of gene(s):		C. reinhardtii
Relevant mutations/deletions:		N/A
Fusion proteins or tags:		GFP
Terminal:		C terminal on insert
Vector backbone:		FUGW (Search Vector Database)
Backbone manufacturer:		Lois/Baltimore
Type of vector:		Mammalian expression,Lentiviral,Mouse Targeting
Backbone size (bp):		9115
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		5' GCACCTTTTGAAATGTAATC 3' (List of Sequencing Primers)
3' Sequencing primer:		5' AGAATACCAGTCAATCTTTCAC 3'
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		Stbl3
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

318 - 605

CMV_immearly_promoter

239 - 815

CMV_fwd_primer

772 - 792

HIV-1_5_LTR

835 - 1015

truncHIV-1_3_LTR

835 - 1015

HIV-1_psi_pack

1126 - 1170

RRE

1680 - 1913

ORF frame 1

1558 - 2445

cPPT

2444 - 2459

hUbC_promoter

2618 - 3771

ORF frame 3

3795 - 5459

EGFP_N_primer

4806 - 4785

EGFP

4752 - 5456

EGFP_C_primer

5393 - 5414

WPRE

5486 - 6073

pBluescriptKS_primer

6092 - 6076

ORF frame 3

5574 - 6623

cPPT

6260 - 6275

U3PPT

6260 - 6281

HIV-1_5_LTR

6597 - 6777

truncHIV-1_3_LTR

6597 - 6777

BGH_rev_primer

6820 - 6803

bGH_PA_terminator

6806 - 7033

f1_origin

7096 - 7402

pBABE_3_primer

7536 - 7516

SV40_enhancer

7737 - 7522

SV40_promoter

7534 - 7802

SV40_origin

7701 - 7778

SV40pro_F_primer

7763 - 7782

EM7_promoter

7896 - 7963

bleo

7964 - 8335

sh_ble

7964 - 8338

SV40_PA_terminator

8471 - 8590

EBV_rev_primer

8559 - 8578

M13_reverse_primer

8652 - 8634

M13_pUC_rev_primer

8673 - 8651

lac_promoter

8716 - 8687

pBR322_origin

9644 - 9025

ORF frame 1

10659 - 9799

Ampicillin

10659 - 9799

AmpR_promoter

10729 - 10701

Unique restriction sites

NdeI		487
NotI		1525
BamHI		3783
AgeI		4730
EcoRI		5460
KpnI		6210
MscI		7967
FspI		10094

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22051" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.