Addgene - LCAG-Halo-GFP Plasmid Data

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Plasmid 22052: LCAG-Halo-GFP

Gene/insert name:		Halo-GFP
Alternative names:		Halo
		NpHR
		halorhodopsin
Insert size (bp):		1617
GenBank/Entrez ID of insert:		EF537649
Species of gene(s):		N. pharaonis
Relevant mutations/deletions:		N. pharaonis halorhodopsin is mammalian codon-optimized
Fusion proteins or tags:		GFP
Terminal:		C terminal on insert
Vector backbone:		LB2 (Search Vector Database)
Backbone manufacturer:		Patrick Stern
Type of vector:		Mammalian expression,Lentiviral,Mouse Targeting
Backbone size (bp):		8958
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		5' ATTGATTTGATACCGCGGGC 3' (List of Sequencing Primers)
3' Sequencing primer:		5' CAAAGAGATAGCTTGGTATT 3'
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		Stbl3
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

lac_promoter

525 - 554

M13_pUC_rev_primer

568 - 590

M13_reverse_primer

589 - 607

HIV-1_5_LTR

869 - 1049

truncHIV-1_3_LTR

869 - 1049

HIV-1_psi_pack

1160 - 1204

RRE

1720 - 1953

ORF frame 2

1598 - 2485

cPPT

2484 - 2499

CAG_enhancer

3113 - 3400

ORF frame 1

4383 - 3775

pCAG_F_primer

4718 - 4737

ORF frame 2

4805 - 6409

EGFP_N_primer

5756 - 5735

EGFP

5702 - 6406

EGFP_C_primer

6343 - 6364

WPRE

6606 - 7193

ORF frame 2

6707 - 7348

pBluescriptKS_primer

7212 - 7196

cPPT

8213 - 8228

U3PPT

8213 - 8234

bGH_PA_terminator

8352 - 8567

HIV-1_5_LTR

8581 - 8761

truncHIV-1_3_LTR

8581 - 8761

pBR322_origin

9427 - 8808

ORF frame 3

10442 - 9582

Ampicillin

10442 - 9582

AmpR_promoter

10512 - 10484

Unique restriction sites

NcoI		3408
XbaI		4663
SmaI		4782
XhoI		4788
BamHI		4793
StuI		4941
AgeI		5680
EcoRI		6410
FspI		9877

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22052" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.