Addgene - FCK-Arch-CFP Plasmid Data

Browse > Edward Boyden > Chow et al > FCK-Arch-CFP

Price: $65.00

Plasmid Links

Related Plasmids

Edward Boyden Lab Plasmids

This is commonly
requested with

Print Friendly Email

Plasmid 22221: FCK-Arch-CFP

Gene/insert name:		Arch
Alternative names:		Arch-CFP
		archaerhodopsin-3
		H. sodomense archaerhodopsin-3
Insert size (bp):		1518
Species of gene(s):		H. sodomense
Relevant mutations/deletions:		NO
Fusion proteins or tags:		CFP
Terminal:		C terminal on insert
Vector backbone:		FCK(1.3)GW (Search Vector Database)
Backbone manufacturer:		Pavel Osten
Type of vector:		Mammalian expression,Lentiviral
Backbone size (bp):		9227
Cloning site 5':		Bam HI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		atgactgagaccctcccacccgtg actgaaagcgccgt (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		Stbl3
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

318 - 605

CMV_immearly_promoter

239 - 815

CMV_fwd_primer

772 - 792

HIV-1_5_LTR

835 - 1015

truncHIV-1_3_LTR

835 - 1015

HIV-1_psi_pack

1126 - 1170

RRE

1680 - 1913

ORF frame 1

1558 - 2445

cPPT

2444 - 2459

ORF frame 1

3745 - 5412

EGFP_N_primer

4759 - 4738

ECFP

4705 - 5409

EGFP_C_primer

5346 - 5367

WPRE

5439 - 6026

pBluescriptKS_primer

6045 - 6029

ORF frame 1

5527 - 6576

cPPT

6213 - 6228

U3PPT

6213 - 6234

HIV-1_5_LTR

6550 - 6730

truncHIV-1_3_LTR

6550 - 6730

BGH_rev_primer

6773 - 6756

bGH_PA_terminator

6759 - 6986

f1_origin

7049 - 7355

pBABE_3_primer

7489 - 7469

SV40_enhancer

7690 - 7475

SV40_promoter

7487 - 7755

SV40_origin

7654 - 7731

SV40pro_F_primer

7716 - 7735

EM7_promoter

7849 - 7916

bleo

7917 - 8288

sh_ble

7917 - 8291

SV40_PA_terminator

8424 - 8543

EBV_rev_primer

8512 - 8531

M13_reverse_primer

8605 - 8587

M13_pUC_rev_primer

8626 - 8604

lac_promoter

8669 - 8640

pBR322_origin

9597 - 8978

ORF frame 2

10612 - 9752

Ampicillin

10612 - 9752

AmpR_promoter

10682 - 10654

Unique restriction sites

SpeI		252
NdeI		487
NarI		1019
NotI		1525
XbaI		2651
BamHI		3895
AgeI		4683
EcoRI		5413
XhoI		6041
KpnI		6163
MscI		7920
FspI		10047

Article: High-performance genetically targetable optical neural silencing by light-driven proton pumps. Chow BY et al. (Nature. 2010 Jan 7. 463(7277):98-102. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22221" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.