Addgene - AAV-FLEX-Arch-GFP Plasmid Data

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Edward Boyden Lab Plasmids

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Plasmid 22222: AAV-FLEX-Arch-GFP

Gene/insert name:		Arch
Alternative names:		Arch-GFP
		archaerhodopsin-3
		H. sodomense archaerhodopsin-3
Insert size (bp):		1511
Species of gene(s):		H. sodomense
Relevant mutations/deletions:		no
Fusion proteins or tags:		GFP
Terminal:		C terminal on insert
Vector backbone:		AAV-FLEX (Search Vector Database)
Backbone manufacturer:		Scott Sternson
Type of vector:		Mammalian expression,Cre/Lox,adeno-associated virus
Backbone size (bp):		5340
Cloning site 5':		KpnI
Site destroyed during cloning:		No
Cloning site 3':		BsrGI
Site destroyed during cloning:		No
5' Sequencing primer:		CCCATAACTTCGTATAATGTATGC (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use recombinase-free E. coli (e.g., Invitrogen's Stbl3), grow at 30C
Sequence:		View sequence
Plasmid Provided In:		Stbl3
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

pBR322_origin

786 - 167

ORF frame 2

1801 - 941

Ampicillin

1801 - 941

AmpR_promoter

1871 - 1843

f1_origin

2370 - 2064

pCAG_F_primer

3542 - 3561

loxP

3629 - 3596

loxP

3596 - 3629

loxP

3725 - 3692

EGFP_C_primer

3804 - 3783

EGFP

4448 - 3741

EGFP_N_primer

4391 - 4412

ORF frame 3

3705 - 5264

ORF frame 3

5243 - 3738

loxP

5299 - 5266

loxP

5266 - 5299

loxP

5370 - 5403

WPRE

5447 - 6034

EBV_rev_primer

6106 - 6087

SV40_PA_terminator

6192 - 6073

Unique restriction sites

NdeI		2769
XbaI		3487
AgeI		4462
PstI		5227
KpnI		5254
BglII		5352
BstBI		5419
HindIII		5433
SalI		6043
NotI		6054
EagI		6054
HpaI		6197

Article: High-performance genetically targetable optical neural silencing by light-driven proton pumps. Chow BY et al. (Nature. 2010 Jan 7. 463(7277):98-102. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22222" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.