Addgene - FCK-Mac-GFP Plasmid Data

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Plasmid 22223: FCK-Mac-GFP

Gene/insert name:		Mac
Alternative names:		Mac-GFP
		Leptosphaeria maculans opsin
		opsin
Insert size (bp):		1683
Species of gene(s):		L. maculans
Relevant mutations/deletions:		NO
Fusion proteins or tags:		GFP
Terminal:		C terminal on insert
Vector backbone:		FCK(1.3)GW (Search Vector Database)
Backbone manufacturer:		Pavel Osten
Type of vector:		Mammalian expression,Lentiviral
Backbone size (bp):		9227
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		atgactgagaccctcccacccgtg actgaaagcgccgt (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		Stbl3
Principal Investigator:		Edward Boyden
Terms and Licenses:		MTA

Comments: PLEASE CONTACT ED BOYDEN (esb@media.mit.edu) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

318 - 605

CMV_immearly_promoter

239 - 815

CMV_fwd_primer

772 - 792

HIV-1_5_LTR

835 - 1015

truncHIV-1_3_LTR

835 - 1015

HIV-1_psi_pack

1126 - 1170

RRE

1680 - 1913

ORF frame 1

1558 - 2445

cPPT

2444 - 2459

ORF frame 1

3745 - 5577

EGFP_N_primer

4924 - 4903

EGFP

4870 - 5574

EGFP_C_primer

5511 - 5532

WPRE

5604 - 6191

pBluescriptKS_primer

6210 - 6194

ORF frame 1

5692 - 6741

cPPT

6378 - 6393

U3PPT

6378 - 6399

HIV-1_5_LTR

6715 - 6895

truncHIV-1_3_LTR

6715 - 6895

BGH_rev_primer

6938 - 6921

bGH_PA_terminator

6924 - 7151

f1_origin

7214 - 7520

pBABE_3_primer

7654 - 7634

SV40_enhancer

7855 - 7640

SV40_promoter

7652 - 7920

SV40_origin

7819 - 7896

SV40pro_F_primer

7881 - 7900

EM7_promoter

8014 - 8081

bleo

8082 - 8453

sh_ble

8082 - 8456

SV40_PA_terminator

8589 - 8708

EBV_rev_primer

8677 - 8696

M13_reverse_primer

8770 - 8752

M13_pUC_rev_primer

8791 - 8769

lac_promoter

8834 - 8805

pBR322_origin

9762 - 9143

ORF frame 2

10777 - 9917

Ampicillin

10777 - 9917

AmpR_promoter

10847 - 10819

Unique restriction sites

SpeI		252
NdeI		487
NotI		1525
XbaI		2651
BamHI		3895
AgeI		4848
EcoRI		5578
SacII		6105
XhoI		6206
KpnI		6328
FspI		10212

Article: High-performance genetically targetable optical neural silencing by light-driven proton pumps. Chow BY et al. (Nature. 2010 Jan 7. 463(7277):98-102. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 22223" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.