Addgene - pcdna3.1 hciap2 Plasmid Data

Gene/insert name:		cIAP2
Insert size (bp):		1800
Gene/insert aliases:		BIRC3, AIP1, API2, MIHC, CIAP2, HAIP1, HIAP1, MALT2, RNF49, c-IAP2
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		myc
Terminal:		N terminal on backbone
Vector backbone:		pcDNA3.1 (Search Vector Database)
Backbone manufacturer:		Invitrogen
Type of vector:		Mammalian expression,Luciferase
Backbone size (bp):		5400
Cloning site 5':		Xho1
Site destroyed during cloning:		No
Cloning site 3':		BamH1
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
3' Sequencing primer:		SP6
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Jon Ashwell
Terms and Licenses:		MTA, Luciferase LULL

Comments: There is a N100D change in the amino acid sequence, according to the author this mutation does not affect protein function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Li X et al. (Nature 2002 Mar 21;416(6878):345-7. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 8338" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.