Addgene - p236 pPr-CREM Plasmid Data

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Plasmid 8404: p236 pPr-CREM

Gene/insert name:		probasin driven CREM
Alternative names:		modified Cre
Insert size (bp):		Unknown
Relevant mutations/deletions:		modified Cre
Vector backbone:		pbeta-gal-Basic (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression,Cre/Lox
Backbone size (bp):		7500
Cloning site 5':		XhoI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Jeffrey Green
Terms and Licenses:		MTA

Comments: 428 bp probasin promoter fragment from prPb-tva950 (Pam Schwartzenberg, NCI), CREM from p210 pCMV-CREM, coding sequence for beta-gal removed.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ et al. (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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