Addgene - CMV-H3.3-YFP Plasmid Data

Browse > Kami Ahmad > Janicki et al > CMV-H3.3-YFP

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NCBI: His3.3A

This is commonly
requested with

CMV-dH3-YFP

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Plasmid 8693: CMV-H3.3-YFP

Gene/insert name:		H3.3A
Alternative names:		His3.3A
Insert size (bp):		1200
Gene/insert aliases:		His3.3A, H3.3, His3.3, PH3, Dmel\CG5825, CG5825
Species of gene(s):		D. melanogaster (fly)
Fusion proteins or tags:		YFP
Terminal:		C terminal on insert
Vector backbone:		na (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		9800
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		Bgl II
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Kami Ahmad
Terms and Licenses:		MTA

Comments: Ahmad lab #k339.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

pBR322_origin

840 - 233

ORF frame 2

1855 - 995

Ampicillin

1855 - 995

AmpR_promoter

1925 - 1897

pGEX_3_primer

2106 - 2084

5xGal4_DBD

3038 - 3131

HS_promoter

3150 - 3377

CAG_enhancer

3568 - 3855

CMV_immearly_promoter

3489 - 4105

CMV_fwd_primer

4022 - 4042

T7_promoter

4116 - 4134

EGFP_N_primer

4685 - 4664

ORF frame 2

4190 - 5365

EYFP

4619 - 5326

EGFP_C_primer

5272 - 5293

SV40_int

5454 - 5469

SV40_3_splice

5475 - 5522

SV40_PA_terminator

6098 - 6229

EBV_rev_primer

6186 - 6205

ORF frame 2

7586 - 8203

Unique restriction sites

FspI		1290
EcoRI		3404
XhoI		3459
SpeI		3502
XbaI		4154
EagI		4601
BglII		5354
HpaI		6094
StuI		6244
AflII		6829
SmaI		8403
NruI		8743
ClaI		8988
EcoRV		9233

Article: From silencing to gene expression: real-time analysis in single cells. Janicki SM et al. (Cell 2004 Mar 5;116(5):683-98. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 8693" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.