Addgene - CMV-dH3-YFP Plasmid Data

Browse > Kami Ahmad > Janicki et al > CMV-dH3-YFP

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His3:CG316... plasmids

Kami Ahmad Lab Plasmids

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NCBI: His3:CG316...

This is commonly
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CMV-H3.3-YFP

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Plasmid 8694: CMV-dH3-YFP

Gene/insert name:		H3
Alternative names:		Histone H3
Insert size (bp):		1174
Gene/insert aliases:		His3:CG31613, CG31613, Dmel\CG31613, His3
Species of gene(s):		D. melanogaster (fly)
Fusion proteins or tags:		YFP
Terminal:		C terminal on insert
Vector backbone:		na (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		9800
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		Bgl II
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Kami Ahmad
Terms and Licenses:		MTA

Comments: Ahmad lab #k245.3

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

pBR322_origin

840 - 233

ORF frame 2

1855 - 995

Ampicillin

1855 - 995

AmpR_promoter

1925 - 1897

pGEX_3_primer

2106 - 2084

5xGal4_DBD

3038 - 3131

HS_promoter

3150 - 3377

CAG_enhancer

3568 - 3855

CMV_immearly_promoter

3489 - 4105

CMV_fwd_primer

4022 - 4042

T7_promoter

4116 - 4134

EGFP_N_primer

4659 - 4638

ORF frame 3

4164 - 5339

EYFP

4593 - 5300

EGFP_C_primer

5246 - 5267

SV40_int

5428 - 5443

SV40_3_splice

5449 - 5496

SV40_PA_terminator

6072 - 6203

EBV_rev_primer

6160 - 6179

ORF frame 3

7560 - 8177

Unique restriction sites

FspI		1290
EcoRI		3404
XhoI		3459
SpeI		3502
XbaI		4154
NheI		4449
NotI		4575
EagI		4575
BglII		5328
HpaI		6068
StuI		6218
AflII		6803
SmaI		8377
NruI		8717
ClaI		8962
EcoRV		9207

Article: From silencing to gene expression: real-time analysis in single cells. Janicki SM et al. (Cell 2004 Mar 5;116(5):683-98. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 8694" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.