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Browse > Kami Ahmad > Ahmad et al > pPgCdH3
Plasmid 8695: pPgCdH3
Gene/insert name: cid promoter, H3
Alternative names: H3
cid
Insert size (bp): Unknown
Gene/insert aliases: His3:CG31613, CG31613, Dmel\CG31613, His3
Species of gene(s): D. melanogaster (fly)
Fusion proteins or tags: GFP
Terminal: C terminal on insert
Vector backbone: na
(Search Vector Database)
Type of vector: Insect expression
Backbone size (bp): Unknown
5' Sequencing primer: na  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Sequence:View sequence
Plasmid Provided In:DH5a
Principal Investigator:Kami Ahmad
Terms and Licenses:MTA

Comments: Ahmad lab #k91.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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Selected features
pBR322_origin 841 - 234
ORF frame 3 1856 - 996
Ampicillin 1856 - 996
AmpR_promoter 1926 - 1898
pGEX_3_primer 2107 - 2085
5xGal4_DBD 3039 - 3132
HS_promoter 3151 - 3378
HS_promoter 3614 - 3841
GFP_R_primer 4791 - 4763
GFPfusionREV_primer 4826 - 4807
ORF frame 2 4307 - 5449
GFP(0-717) 4733 - 5449
GFP_F_primer 5386 - 5406
SV40_int 5538 - 5553
SV40_3_splice 5559 - 5606
SV40_PA_terminator 6182 - 6313
EBV_rev_primer 6270 - 6289
ORF frame 2 7670 - 8287
Unique restriction sites

FspI 1291
XhoI 3460
XbaI 3883
NheI 4592
EagI 4718
HpaI 6178
StuI 6328
SmaI 8487
EcoRV 9317

Article: Centromeres are specialized replication domains in heterochromatin. Ahmad K et al. (J Cell Biol 2001 Apr 2;153(1):101-10. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 8695" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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