Addgene - HS-H3.3A d436-GFP Plasmid Data

Browse > Kami Ahmad > Ahmad et al > HS-H3.3A d436-GFP

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NCBI: His3.3A

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Plasmid 8697: HS-H3.3A d436-GFP

Gene/insert name:		H3.3A truncated
Alternative names:		H3.3^core
		His3.3A
Insert size (bp):		315
Gene/insert aliases:		His3.3A, H3.3, His3.3, PH3, Dmel\CG5825, CG5825
Species of gene(s):		D. melanogaster (fly)
Relevant mutations/deletions:		Deletion of amino acids 4-36.
Fusion proteins or tags:		GFP
Terminal:		C terminal on backbone
Vector backbone:		na (Search Vector Database)
Type of vector:		Insect expression
Backbone size (bp):		10200
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		EagI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
3' Sequencing primer:		CCATCTAATTCAACAAGAATTGGG ACAAC
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Kami Ahmad
Terms and Licenses:		MTA

Comments: Same as H3.3^core. Ahmad lab #k58.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

pBR322_origin

840 - 233

ORF frame 2

1855 - 995

Ampicillin

1855 - 995

AmpR_promoter

1925 - 1897

pGEX_3_primer

2106 - 2084

5xGal4_DBD

3038 - 3131

HS_promoter

3150 - 3377

HS_promoter

3613 - 3840

GFP_R_primer

4270 - 4242

GFPfusionREV_primer

4305 - 4286

ORF frame 3

3876 - 4928

GFP(0-717)

4212 - 4928

GFP_F_primer

4865 - 4885

SV40_int

5017 - 5032

SV40_3_splice

5038 - 5085

SV40_PA_terminator

5661 - 5792

EBV_rev_primer

5749 - 5768

ORF frame 3

7149 - 7766

Unique restriction sites

FspI		1290
AatII		1990
EcoRI		3404
XhoI		3459
XbaI		3882
EagI		4197
HpaI		5657
StuI		5807
NarI		7009
SmaI		7966
EcoRV		8796

Article: The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly. Ahmad K et al. (Mol Cell 2002 Jun;9(6):1191-200. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 8697" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.