Addgene - 1036 pcDNA3 Myr HA Akt1 Plasmid Data

Gene/insert name:		Akt1
Alternative names:		Akt
Insert size (bp):		1500
Gene/insert aliases:		AKT1, AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		myr
Terminal:		N terminal on backbone
Fusion proteins or tags:		HA
Terminal:		N terminal on backbone
Vector backbone:		pcDNA3 (Search Vector Database)
Backbone manufacturer:		Invitrogen
Type of vector:		Mammalian expression
Backbone size (bp):		5446
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		CMV-F (List of Sequencing Primers)
3' Sequencing primer:		BGH-rev
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		William Sellers
Terms and Licenses:		MTA

Comments: The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Ramaswamy S et al. (Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 9008" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.