This kit contains 288 vectors and includes both a general description and the full predicted DNA sequence for each vector. Among these vectors are:

• lacZ and/or GFP fusion vectors with higher expression efficiency than many earlier C. elegans vectors. These include vectors with coding region for blue-shifted GFP, cyan-shifted GFP, yellow-shifted GFP, and other GFP protein variants.
• Vectors for tagging C. elegans exons and introns with GFP.
• Control plasmids for RNA-mediated interference.
• Hairpin sequences to test promoters for ability to trigger RNAi in-vivo.
• And more...

The vectors are divided into 3 plates (96 samples each):

• 1995 plate (blue colored caps)
  Plate Map, Documentation (PDF)

• 1997 plate (yellow colored caps)
  Plate Map, Documentation (PDF)

• 1999 plate (green colored caps)
  Plate Map, Documentation (PDF)

• 2005 vectors: H1 through H12 of the 1995 plate, H3 through H12 of the 1999 plate, and E8 and F7 of the 1999 plate are 24 new vectors made available in 2005. These vectors were not available in the 1995 and 1999 vector sets previously distributed by the Fire lab. Documentation (PDF).

Other Documentations:

• PDF file of all plasmid information and maps (8 MB, no sequences).

• Excel file summarizing plasmid features, sequences, and plate locations (2.6 MB).

• Zip file containing a folder that includes plasmid features and sequences for each plasmid as individual files (774 KB). Browse this folder here.

• Text file containing all sequences in GenBank format (3 MB).

• Plasmid information and sequences are also packaged into a Hypercard application called DNA Janitor (472 KB zip/StuffIt). DNA Janitor, written by Dr. Fire, provides interfaces for browsing and searching plasmid information. This application requires a HyperCard viewer (Download from Apple) to run on either MacOS X Classic Mode, or MacOS 9 or earlier.

In the documentations, plasmids may be referred by their mini-prep number (prefix pPD), or ligation number (prefix L). Please consult the above Excel file for translation between these naming schemes. On this website, you may search for a plasmid using either number.

You may order individual plasmids from the kit as well as the entire kit. Addgene does not sell worm strains.

Individual Plasmids: Addgene charges $40 for each vector, plus shipping and handling. This is a reduced price courtesy of Dr. Fire.

Addgene ships individual plasmids as bacterial stabs at room temperature.

The Entire Kit: Addgene charges $375 for the entire kit, plus shipping and handling.

Within the continental United States, Addgene ships the kit as 288 individually capped tubes of bacterial glycerol stocks, in 96-well format, on dry ice. Samples should be frozen at -80 degrees Celsius immediately upon arrival.

Addgene now ships the kit as glycerol stocks in microtiter plates to scientists outside of the United States. Scientists from China will receive 96-well FTA/Clonesaver cards.

To place an order, please Login or Register a new account first. After you have selected a plasmid of interest, click on the Add to Cart button at the top of the plasmid information page. To order the whole kit, click on the Order Kit button at the top of this page. For more information, please consult the Frequently Asked Questions.

The following instructions are included with the kit:

• General Instructions (PDF)
• Instructions for FTA/Clonesaver Cards (PDF)

Handling

If you have received bacterial stabs, Addgene recommends that you store this vial at 4 degrees Celsius for no longer than 2 weeks. If you have received plasmid DNA on filter paper, the plasmid is stable at room temperature for years.

Samples in glycerol stocks should be stored at -80 degrees Celsius immediately upon arrival. You should also minimize the number of freeze-thaw cycles for these samples.