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Zinc Finger Plasmids
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Nuclease Expression Vectors
Zinc Finger Consortium: Nuclease Expression Vectors
This page lists plasmid vectors designed to express nucleases in
different cell types. Zinc finger arrays created using ZFC reagents
for either OPEN or modular assembly can be easily transferred in-frame
to any of these vectors using unique XbaI and BamHI
cloning sites.
Vectors encoding wild-type FokI nuclease domains:
- pST1374
(for expression in mammalian cells or zebrafish)
- pDW1775
(for expression in plants)
Vectors encoding heterodimeric FokI nuclease domains:
-
pMLM290
(for expression of a "+" heterodimeric FokI domain. Miller et al.,
Nat Biotech 2007) in mammalian cells or zebrafish
(can be used as a substitute for pMLM335 described in Foley et al.,
PLoS One 2009)
-
pMLM292
(for expression of a "-" heterodimeric FokI domain. Miller et al.,
Nat Biotech 2007) in mammalian cells or zebrafish (can be
used as a substitute for pMLM336 described in Foley et al., PLoS
One 2009)
