This library consists of 192 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF. Academic and non-profit laboratories may request these vectors from Addgene for research and educational use.

These plasmids have been described in

Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene.
Boehm, Zhao, Yao et al. Cell 129(6):1065-1079, 2007. PubMed 17574021

This kit includes only the kinase library clones from the Boehm, Zhao, Yao et al. publication. All other plasmids from the publication can be found here.

Distribution

The kit is $650, plus shipping and handling. This kit will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80 degrees Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

The Myristoylated Kinase Library plasmids are divided into and distributed as two plates:

Download Information and Plate Map PDF (included with shipment).

Browse contents of the library by plate: Plate 1, Plate 2.

Browse contents of the library by gene name.

 
Note that Addgene uses UPS for shipments and can ship dry ice to most countries, excluding China. Scientists from China interested in obtaining this kit should contact help@addgene.org.