| Gene/insert name: | | huntingtin, exon 1, 16 glutamines | |
| Alternative names: | | huntingtin (Huntington disease) | |
| Insert size (bp): | | 279 | |
| GenBank/Entrez ID of insert: | | NM_002111 | |
| Gene/insert aliases: | | HTT, HD, IT15 | |
| Species of gene(s): | | H. sapiens (human)
| |
| Relevant mutations/deletions: | | huntingtin exon 1 with 16 glutamines,
vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together. | |
| Fusion proteins or tags: | | Trx | |
| Terminal: | | N terminal on backbone | |
| Fusion proteins or tags: | | His | |
| Terminal: | | C terminal on insert | |
| Fusion proteins or tags: | | His (out of frame) | |
| Terminal: | | C terminal on backbone | |
| Vector backbone: | | pET-32a
(Search Vector Database) | |
| Backbone manufacturer: | | Novagen | |
| Type of vector: | | Bacterial expression | |
| Backbone size (bp): | | 5900 | |
| Cloning site 5': | | NcoI | |
| Site destroyed during cloning: | | No | |
| Cloning site 3': | | BamHI | |
| Site destroyed during cloning: | | No | |
| 5' Sequencing primer: | | T7 promoter (List of Sequencing Primers) | |
| 3' Sequencing primer: | | T7 terminator | |
| Bacteria resistance: | | Ampicillin | |
| High or low copy: | | Unknown | |
| Grow in standard E. coli @ 37C: | | Yes | |
| If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: | | R. Myers | |
| Sequence: | | View sequence |
| Plasmid Provided In: | | DH5a |
| Principal Investigator: | | Pamela J. Bjorkman |
| Terms and Licenses: | | MTA |
Article: Inaugural Article: A linear lattice model for polyglutamine in CAG-expansion diseases. Bennett MJ et al. (Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9.
Pubmed)
Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.
Also, please include the text "Addgene plasmid 11487" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.
