Addgene - pAcUW31-M10.5/human b2m Plasmid Data

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H2-M10.4 plasmids

Pamela Bjorkman Lab Plasmids

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AY211081

NCBI: H2-M10.4

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Plasmid 11521: pAcUW31-M10.5/human b2m

Gene/insert name:		M10.5
Alternative names:		M10.5 ectodomain
		major histocompatibility complex class Ib M10.5
Insert size (bp):		948
GenBank/Entrez ID of insert:		AY211081
Gene/insert aliases:		H2-M10.4, 15H
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		His
Terminal:		C terminal on insert
Vector backbone:		pAcUW31 (Search Vector Database)
Backbone manufacturer:		BD Biosciences
Type of vector:		Insect expression
Backbone size (bp):		8532
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		BamHI
Site destroyed during cloning:		No
5' Sequencing primer:		5' CCg gAT CTg ATC ATg gAg ATA A 3' (List of Sequencing Primers)
3' Sequencing primer:		5' TCg CTC TAA CAT ACC ACC CT 3'
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		C. Dulac
Plasmid Provided In:		DH5a
Principal Investigator:		Pamela J. Bjorkman
Terms and Licenses:		MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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Article: Structure of a pheromone receptor-associated MHC molecule with an open and empty groove. Olson R et al. (PLoS Biol. 2005 Aug . 3(8):e257. Pubmed)

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