Addgene - pAcUW51-gE2 Plasmid Data

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Plasmid 11621: pAcUW51-gE2

Gene/insert name:		HSV-1 glycoprotein E
Alternative names:		HSV-1 gE
Insert size (bp):		1233
Species of gene(s):		Herpes simplex virus type I KOS strain
Relevant mutations/deletions:		deleted amino acids 391-550
Fusion proteins or tags:		His
Terminal:		C terminal on insert
Vector backbone:		pAcUW51 (Search Vector Database)
Backbone manufacturer:		BD Biosciences
Type of vector:		Insect expression
Backbone size (bp):		5863
Cloning site 5':		BglII
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		CGGACCTTTAATTCAACCCAAC (List of Sequencing Primers)
3' Sequencing primer:		TTGACACCAGACCAACTGGT
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Pamela J. Bjorkman
Terms and Licenses:		MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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Article: pH dependence and stoichiometry of binding to the Fc region of IgG by the herpes simplex virus Fc receptor gE-gI. Sprague ER et al. (J Biol Chem. 2004 Apr 2. 279(14):14184-93. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11621" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.