Addgene - pCB6-HFE-EGFP Plasmid Data

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Q30201

NCBI: HFE

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Plasmid 12104: pCB6-HFE-EGFP

Gene/insert name:		HFE
Alternative names:		hemochromatosis protein
		MHC class I-like protein HFE
		hereditary hemochromatosis protein HLA-H
Insert size (bp):		1044
GenBank/Entrez ID of insert:		Q30201
Gene/insert aliases:		HFE, HH, HFE1, HLA-H, MVCD7, MGC103790, dJ221C16.10.1
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		EGFP
Terminal:		C terminal on insert
Vector backbone:		pCB6 (Search Vector Database)
Backbone manufacturer:		Ira Mellman, Yale University
Type of vector:		Mammalian expression
Backbone size (bp):		Unknown
Cloning site 5':		unknown
Site destroyed during cloning:		Unknown
Cloning site 3':		unknown
Site destroyed during cloning:		Unknown
5' Sequencing primer:		unknown (List of Sequencing Primers)
3' Sequencing primer:		unknown
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Pamela J. Bjorkman
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: HFE and transferrin directly compete for transferrin receptor in solution and at the cell surface. Giannetti AM et al. (J Biol Chem. 2004 Jun 11. 279(24):25866-75. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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