Addgene - pAcGP67A-TfR Plasmid Data

Browse > Pamela J. Bjorkman > Lebron et al > pAcGP67A-TfR

Price: $65.00

Plasmid Links

Related Plasmids

Pamela Bjorkman Lab Plasmids

Other Links

NP_003225

NCBI: TFRC

TFRC antibodies

Print Friendly Email

Plasmid 12130: pAcGP67A-TfR

Gene/insert name:		transferrin receptor
Alternative names:		TfR
Insert size (bp):		2000
GenBank/Entrez ID of insert:		NP_003225
Gene/insert aliases:		TFRC, TFR, CD71, TFR1, TRFR
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		The portion of the human TfR gene encoding residues 121-760 were fused 3' to a gene segment encoding the hydrophobic leader peptide from the baculovirus protein gp67, a 6X-His tag, and a factor Xa cleavage site in a modified version of the pAcG67A expression vector.
Fusion proteins or tags:		leader peptide from baculovirus protein gp67
Terminal:		N terminal on insert
Fusion proteins or tags:		6X-His tag
Terminal:		N terminal on insert
Fusion proteins or tags:		Factor Xa cleavage site
Terminal:		N terminal on insert
Vector backbone:		pAcGP67A (Search Vector Database)
Backbone manufacturer:		Pharmingen
Type of vector:		Insect expression
Backbone size (bp):		9761
Cloning site 5':		unknown
Site destroyed during cloning:		Unknown
Cloning site 3':		unknown
Site destroyed during cloning:		Unknown
5' Sequencing primer:		5' AAT TTAA AAT gAT AAC CAT CTC gC 3' (List of Sequencing Primers)
3' Sequencing primer:		5' gAA gCg ggT CCA AgT TTC CCT g 3'
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Pamela J. Bjorkman
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Article: Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor. Lebron JA et al. (Cell. 1998 Apr 3. 93(1):111-23. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12130" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.