Addgene - pAcGP67A - murine TfR Plasmid Data

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Plasmid 12392: pAcGP67A - murine TfR

Gene/insert name:		transferrin receptor
Alternative names:		TfR
Insert size (bp):		2000
GenBank/Entrez ID of insert:		NM_011638
Gene/insert aliases:		Tfrc, TFR, p90, CD71, TFR1, Trfr, Mtvr1, Mtvr-1, AI195355, AI426448, AU015758, 2610028K12Rik, E430033M20Rik
Species of gene(s):		M. musculus (mouse)
Relevant mutations/deletions:		See comments section
Fusion proteins or tags:		leader peptide from baculovirus protein gp67
Terminal:		N terminal on insert
Fusion proteins or tags:		6X-His tag
Terminal:		N terminal on insert
Fusion proteins or tags:		Factor Xa cleavage site
Terminal:		N terminal on insert
Vector backbone:		pAcGP67A (Search Vector Database)
Backbone manufacturer:		Pharmingen
Type of vector:		Insect expression
Backbone size (bp):		9761
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		EagI
Site destroyed during cloning:		No
5' Sequencing primer:		5' AAT TTAA AAT gAT AAC CAT CTC gC 3' (List of Sequencing Primers)
3' Sequencing primer:		5' gAA gCg ggT CCA AgT TTC CCT g 3'
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		Gerhard Rank, PhD Rotary Bone Marrow Research Laboratories Royal Melbourne Hospital Royal Pde Parkville 3050 VIC Australia
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Pamela J. Bjorkman
Terms and Licenses:		MTA

Comments: The murine TfR sequence bp 367-2292 (of the ORF) was cloned into the pAcGP67A vector. The construct includes a short 3' UTR (past the stop codon at bp 2292). The TfR fragment was PCR amplified to introduce an EcoRI site at the 5' end and an EagI site at the 3' end. This was subsequently cloned into the corresponding EcoRI/EagI sites of pAcGP67A. The murine TfR was fused 3' to a gene segment encoding the hydrophobic leader peptide from the baculovirus protein gp67, a 6X-His tag, and a factor Xa cleavage site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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