Addgene - pET20b-pIgR D1-H6 Plasmid Data

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P01833

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Plasmid 12530: pET20b-pIgR D1-H6

Gene/insert name:		human polymeric immunoglobulin receptor
Alternative names:		human pIgR
Insert size (bp):		350
GenBank/Entrez ID of insert:		P01833
Gene/insert aliases:		PIGR, FLJ22667, MGC125361, MGC125362
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		Lys 1-Val 109
Fusion proteins or tags:		His
Terminal:		C terminal on backbone
Vector backbone:		pET20b (Search Vector Database)
Backbone manufacturer:		Novagen
Type of vector:		Bacterial expression
Backbone size (bp):		3716
Cloning site 5':		Nde I
Site destroyed during cloning:		No
Cloning site 3':		Xho I
Site destroyed during cloning:		No
5' Sequencing primer:		T7 promoter primer (List of Sequencing Primers)
3' Sequencing primer:		T7 terminator primer
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		Roland Strong
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Pamela J. Bjorkman
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Crystal structure of a polymeric immunoglobulin binding fragment of the human polymeric immunoglobulin receptor. Hamburger AE et al. (Structure. 2004 Nov . 12(11):1925-35. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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